Sequencing: Sanger Sequencing, NGS, whole exome, whole transcriptome, RNASeq
Note: Service includes extraction of DNA (following receipt of samples, at least 1ug per sample required), Qubit quantitation, library preparation and QC (Qubit, Bioanalyser), next generation sequencing (Ion Proton, Ion S5, NextSeq2000), data collection, alignment, report generation. Data sent as BAM and BAI files.
Note: Includes sample preparation, library preparation, template and sequencing quantitation, next generation sequencing (Ion Proton, 10-20 million reads per sample), alignment and Quality control. Data sent as BAM and BAI files.
Note: Service includes extraction of RNA (following receipt of samples, at least 1ug per sample required), Qubit quantitation, rRNA depletion (Robominus kit, ERCC spike in control), RNA quantitation and integrity (Agilent Pico chips), library preparation and QC (Qubit, Bioanalyser), sequencing (Life Technologies Ion Total RNASeq Kit, Ion PI Hi-Q Chef Kit, Ion Proton, 4 samples per PI chip (50 or 100bp reads, 10-20 million reads per sample), data collection, alignment, report generation. Data sent as BAM and BAI files.
Please note:
For all sequencing, analysis is dependent on sample/study size and is to be discussed
GST is not applicable to services provided internally (within QUT) but will be added to external transactions.