New MRS Publication: An electrochemical biosensor for the rapid detection of erythropoietin in blood

The QUT Molecular Recognition and Sensors (MRS) group is pleased to announce a new publication authored by our PhD Student Waleed Hassanien.

An electrochemical biosensor for the rapid detection of erythropoietin in blood

, Sivanesan, Arumugam, , & (2018) An electrochemical biosensor for the rapid detection of erythropoietin in blood. Talanta. (In Press)

“the developed method has a strong potential for the sensitive detection of rhuEPO doping in sports”

Abstract

A label free electrochemical detection method for the rapid detection of recombinant human erythropoietin (rhuEPO) has been developed. In this method, we modified the rhuEPO structure for its direct sensing without using a complex signal amplification strategy. The protein was selectively extracted from blood plasma sample using target-specific magnetic beads. After releasing rhuEPO from the magnetic beads, its disulfide bonds were electrochemically reduced and the protein was spontaneously assembled onto a nanostructured gold electrode via Au-S bonds formation. For electrochemical quantification, the reduced protein was desorbed from the electrode surface using differential pulse voltammetry (DPV). The desorption current was proportional to the concentration of rhuEPO in the range 1 pM – 1000 pM. By cross-validating against ELISA, we found a 104.85 ± 3.35% agreement between the results obtained using the electrochemical biosensor and ELISA. Therefore the developed method has a strong potential for the sensitive detection of rhuEPO doping in sports as well as its rapid screening and pathology labs.

 

View on QUT ePrints: https://eprints.qut.edu.au/120144/

View on Publisher’s website: https://www.sciencedirect.com/science/article/pii/S0039914018307537?via%3Dihub