Sequencing – Sanger Sequencing, NGS, WES, Transcriptome, RNASeq
Sanger Sequencing:
SS single (F or R) – $5.00 per sample
SS, PCR cleanup and BDT single (F or R) – $10.50 per sample
SS, PCR cleanup and BDT Dual (F and R) – $17.00 per sample
- Plus Service Fee of $100 for each batch of samples
- Analysis, to be discussed, dependent on sample/study size
Ion Torrent NGS:
Library preparation – $180 per sample
NGS 314 chip (200 and 400bp; 2-10 samples) – $550 per sample
NGS 316 chip (200 and 400bp; 8-15 samples) – $850 per sample
NGS 318 chip (200 and 400bp; 15-25 samples) – $1150 per sample
- Plus Service Fee of $100 for each batch of samples
- Analysis, to be discussed, dependent on sample/study size
Whole exome sequencing:
Whole exome sequencing – $1850 per sample (including GST)
Other disorders
- Plus Service Fee of $100 for each batch of samples
- Analysis, to be discussed, dependent on sample/study size
Note:
Service includes extraction of DNA (following receipt of samples, at least 1ug per sample required), Qubit quantitation, library preparation and QC (Qubit, Bioanalyser), next generation sequencing (Ion Proton, 10-20 million reads per sample), data collection, alignment, report generation. Data sent as BAM and BAI files.
Whole Transcriptome: Ion Proton
Ampliseq Whole Transcriptome – $320 per sample
- Plus Service Fee of $100 for each batch of samples
- Analysis, to be discussed, dependent on sample/study size
Note:
Includes sample preparation, library preparation, template and sequencing quantitation, next generation sequencing (Ion Proton, 10-20 million reads per sample), alignment and Quality control. Data sent as BAM and BAI files.
RNASeq, small RNASeq: Ion Proton
RNASeq or smallRNASeq – $820 per sample
Human, mouse, rat samples
- Plus Service Fee of $100 for each batch of samples
- Analysis, to be discussed, dependent on sample/study size
Service includes extraction of RNA (following receipt of samples, at least 1ug per sample required), Qubit quantitation, rRNA depletion (Robominus kit, ERCC spike in control), RNA quantitation and integrity (Agilent Pico chips), library preparation and QC (Qubit, Bioanalyser), sequencing (Life Technologies Ion Total RNASeq Kit, Ion PI Hi-Q Chef Kit, Ion Proton, 4 samples per PI chip (50 or 100bp reads, 10-20 million reads per sample), data collection, alignment, report generation. Data sent as BAM and BAI files.
Please note: GST is not applicable to services provided internally (within QUT) but will be added to external transactions.