The BTM Group specialises in bone tissue histology, with the capacity to process both soft and hard tissue samples using parafin, cryo and more specialised resin histology. Fresh fixed tissue are processed, embedded, sectioned, stained and imaged, all within a single core facility.
- Hard or mineralised tissues is rapidly decalcified before processing for routine paraffin histology and immunohistochemistry.
- Samples are embedded in resin then thin sectioned, or ground sectioned with the high precision EXAKT saw and grinder. This process better preserves tissue morphology and is ideal for bone and metallic implants (dentistry and orthopaedics).
- Samples with labile antigens or processing sensitive components are rapidly frozen and cryosectioned prior to staining.
Using these advanced histological techniques, the results of our pre-clinical models can be evaluated to determine the quality of regenerated tissue in defect site using our 3D printed scaffolds.
Chief Investigators
Publications
- Medeiros Savi, Flavia, Brierly, Gary Ian, Baldwin, Jeremy, Theodoropoulos, Christina, Woodruff, Mia (2017) Comparison of different decalcification methods using rat mandibles as a model. Journal of Histochemistry and Cytochemistry, 65 (12), pp.705-722.
- Poh, Su, Hutmacher, Dietmar, Holzapfel, Boris, Solanki, Anu, Stevens, Molly, Woodruff, Mia (2016) In vitro and in vivo bone formation potential of surface calcium phosphate-coated polycaprolactone and polycaprolactone/bioactive glass composite scaffolds. Acta Biomaterialia, 30, pp.319-333.
- Berner, Arne, Reichert, Johannes, Woodruff, Mia, Saifzadeh, Siamak, Morris, Anthony, Epari, Devakar, Nerlich, Michael, Schuetz, Michael, Hutmacher, Dietmar (2013) Autologous vs. allogenic mesenchymal progenitor cells for the reconstruction of critical-sized segmental tibial bone defects in aged sheep. Acta Biomaterialia, 9 (8), pp.7874-7884.
- Wullschleger, Martin, Steck, Roland, Matthys, Romano, Webster, John, Woodruff, Mia, Epari, Devakar, Ito, Keita, Schuetz, Michael (2013) A new model to study healing of a complex femur fracture with concurrent soft tissue injury in sheep. Open Journal of Orthopedics, 3 (2), pp.62-68.
- Berner, Arne, Woodruff, Mia, Lam, Christopher, Arafat, M.T., Saifzadeh, Siamak, Steck, Roland, Ren, Jiongyu Edward, Nerlich, Michael, Ekaputra, Andrew Krishna, Gibson, Ian, et al. (2014) Effects of scaffold architecture on cranial bone healing. International Journal of Oral and Maxillofacial Surgery, 43 (4), pp.506-513.
- Woodruff, Mia, Lange, Claudia, Chen, Fulin, Fratzl, Peter, Hutmacher, Dietmar (2013) Nano- to macroscale remodeling of functional tissue-engineered bone. Advanced Healthcare Materials, 2 (4), pp.546-551.
- Reichert, Johannes, Cipitria, Amaia, Epari, Devakar, Saifzadeh, Siamak, Krishnakanth, Pushpanjali, Berner, Arne, Woodruff, Mia, Schell, Hanna, Mehta, Manav, Schuetz, Michael, et al. (2012) A tissue engineering solution for segmental defect regeneration in load-bearing long bones. Science Translational Medicine, 4 (141), pp.1-10.
